Simvastatin-activated implant surface promotes osteoblast differentiation in vitro. (February 2014)

• Record Type: Journal Article
• Authors: Walter, Martin Sebastian; Frank, Matthias Johannes; Rubert, Marina; Monjo, Marta; Lyngstadaas, Staale Petter; Haugen, Håvard Jostein
• Is Part Of: Journal of biomaterials applications. Volume 28:Number 6(2014:Feb.)
• Page Start: 897
• Page End: 908
• Publication Date: 2014-02
• Subjects: Anodic oxidation -- bioactivation -- statins -- titanium zirconium -- implant surface analysis -- osteoblast differentiation; Biomedical engineering -- Periodicals; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1177/0885328213486364
• Languages: English
• ISSNs: 0885-3282

Abstract

The bone growth promoting effects of statins suggest that these bioactive molecules can be used to improve the integration of bone-anchored implants. This study aimed at the application of simvastatin with dental implants for use in patients with low bone density. Coin-shaped titanium zirconium samples with grit-blasted and acid-etched surface were coated with simvastatin, using a novel anodic oxidation setup under alkaline conditions. The presence of intact simvastatin attached to the surface was confirmed by infrared spectroscopy. A binding site on the aliphatic O-H group was discovered and the integration of 1 H, 18 O and 12 C in the depth of the surface were observed by secondary ion mass spectroscopy. A simvastatin concentration of about 60 g/cm 2 was found in a release study over 72 h. The simvastatin-coated surfaces promoted alkaline phosphatase, collagen type I and osteocalcin gene expression of MC3T3-E1 cells. This suggested that the demonstrated coating holds potential for use in patients with compromised bone.