Photodynamic therapy of human biliary cancer cell line using combination of phosphorus porphyrins and light emitting diode. Issue 24 (15th December 2017)

• Record Type: Journal Article
• Authors: Matsumoto, Jin; Suzuki, Kou; Yasuda, Masahide; Yamaguchi, Yuya; Hishikawa, Yoshitaka; Imamura, Naoya; Nanashima, Atsushi
• Is Part Of: Bioorganic & medicinal chemistry. Volume 25:Issue 24(2017)
• Page Start: 6536
• Page End: 6541
• Publication Date: 2017-12-15
• Subjects: A absorbance -- B number of living cell in cell well−1 -- CLSM confocal laser scanning microscope -- DMEM Dulbecco's modified Eagle's medium -- DMSO dimethyl sulfoxide -- HeLa human cervix adenocarcinoma -- IC50 half maximal inhibitory concentration -- LD50 lethal dose 50 -- LED light emitting diode -- MTT 3[4, 5-Dimethyl-thiazoyl-2-yl]-2, 5-diphenyltetrazolium bromide -- NOZ human biliary cancer cell line -- PBS phosphate-buffered saline -- PDT photodynamic therapy -- tpp tetraphenylporphyrinato group -- ΦΔ overall quantum yield for generation of 1O2; Photodynamic therapy -- Phosphorus porphyrins -- LED -- Human biliary cancer cell line -- HeLa cell -- IC50; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; Biochemistry -- Periodicals; Chemistry, Clinical -- Periodicals; Chemistry, Organic -- Periodicals; Chimie bio-organique -- Périodiques; Chimie pharmaceutique -- Périodiques; 615.19
• DOI: 10.1016/j.bmc.2017.10.031
• Languages: English
• ISSNs: 0968-0896

Abstract

Graphical abstract: Abstract: A series of phosphorus porphyrin complexes ([(RO)2 P(tpp)]Cl, tpp = tetraphenylporphyrinato group, R = −(CH2 CH2 O)m (CH2 )n H;1a : m = 2, n = 2;1b : m = 2, n = 4;1c : m = 2, n = 6;1d : m = 3, n = 6) were used for the photodynamic therapy (PDT) of human biliary cancer cell line (NOZ) when exposed to the irradiation of light emitting diodes (LEDs). A Dulbecco's modified Eagle's medium (DMEM) containing NOZ cells (2000 cell well −1 ) and1 (0–100 nM) was introduced into a 96-well microplate and incubated for 24 h to accumulate1 into the NOZ cells and to multiply the NOZ cells until the cell number reached 10 4 cells well −1 . After replacing the DMEM medium containing1 with a fresh DMEM medium without1, the plates were irradiated for 30 min at 610 nm. After incubation was performed for 24 h in dark conditions, the cell viability of the NOZ cells was determined using the MTT assay. The half maximum inhibitory concentrations 50 (IC50 ) of1a –1d were found to be in the range of 33.7–58.7 nM for NOZ. These IC50 values for the NOZ were one hundredth the IC50 value (7.57 μM) for mono-l -aspartyl chlorin e6 (laserphyrin®). Thus, it was found that the PDT activity of1a –1d was much higher than the mono-l -aspartyl chlorin e6. Similarly, IC50 vales of1a –1d for HeLa cells were found to be 27.8–52.5 nM. This showed that1a –1d had high photodynamic activity in cancer cells. At the same time, it was speculated that an LED is a useful light source for deactivating the cancer cells because it can excite the sensitizers with peak width in their absorption spectra using the light of the specified wave length with band width of 10–20 nm; LEDs provide a homogeneous light distribution for the target cells.