A colorimetric biosensor based on guanidinium recognition for the assay of protein tyrosine phosphatase 1B and its inhibitors. (2nd November 2017)

• Record Type: Journal Article
• Authors: Lv, Jun; Chen, Tingjun; Yue, Xiquan; Zhou, Jianqiong; Gong, Xiuqing; Zhang, Juan
• Is Part Of: New journal of chemistry. Volume 41:Number 23(2017)
• Page Start: 14414
• Page End: 14419
• Publication Date: 2017-11-02
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/c7nj02918g
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : A new colorimetric biosensor for the assay of PTP1B and its inhibitors based on coordination between RGC/AuNPs and MNPs/APP. Abstract : In this study, a colorimetric biosensor was established for the detection of protein tyrosine phosphatase 1B (PTP1B) and its inhibitor based on the guanidinium recognition assisted coordination of arginine-glycine-cysteine (RGC) modified gold nanoparticles (RGC/AuNPs) with 4-aminophenyl phosphate-functionalized Fe3 O4 magnetic nanoparticles (MNPs/APP). PTP1B can catalyze the cleavage of the phosphate ester bond and the departure of the phosphate acid group from the surface of MNPs/APP, resulting in the loss of the corresponding coordination reactivity. Upon the addition of a solution of RGC/AuNPs, RGC/AuNPs remained in the supernatant solution after magnetic separation and a high absorbance value was observed. Thus, a simple colorimetric biosensor for PTP1B assay was developed. Under the optimized experimental conditions, PTP1B was detected within a linear range of 0.002 U mL −1 to 0.08 U mL −1 with the lowest detection limit of 0.0013 U mL −1 . Moreover, using this proposed biosensor, the inhibition effect of betulinic acid and 6-chloro-3-formyl-7-methylchromone on PTP1B is determined with IC50 values of 9 μM and 15 μM, respectively. Therefore, this new biosensor not only has great potential for PTP1B analysis but also for the detection of its inhibitors.