Quantification of morusin using LC–MS in rat plasma: Application to a pharmacokinetic study. (29th June 2017)

• Record Type: Journal Article
• Authors: Deng, Zhipeng; Sun, Xiaohui; Yang, Shenbao; Zhang, Lei; Sun, Peilu; Teng, Xuepeng; Zhong, Hao
• Is Part Of: Biomedical chromatography. Volume 31:Number 12(2017:Dec.)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-06-29
• Subjects: LC–MS -- morusin -- pharmacokinetic study; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.4021
• Languages: English
• ISSNs: 0269-3879

Abstract

Abstract: A sensitive LC–MS method was developed for the quantification of morusin in rat plasma using praeruptorin C as internal standard. After extraction with diethyl ether, post‐treatment samples were chromatographed on a Hypersil C18 column. An isocratic mobile phase consisting of methanol–water (70:30, v /v) was applied at a flow rate of 0.4 mL/min. Detection was performed via electrospray ionization source with positive ion mode using selected ion monitoring mode at m/z 443.1 for morusin and m/z 451.0 for IS. Acceptable linearity ( r 2 ≥ 0.99) was observed over the concentration range of 1.5–800 ng/mL. This method was successfully applied in the pharmacokinetics study of morusin in rats.