Supramolecular assembly affording a ratiometric two-photon fluorescent nanoprobe for quantitative detection and bioimaging. Issue 12 (17th October 2017)

• Record Type: Journal Article
• Authors: Wang, Peng; Zhang, Cheng; Liu, Hong-Wen; Xiong, Mengyi; Yin, Sheng-Yan; Yang, Yue; Hu, Xiao-Xiao; Yin, Xia; Zhang, Xiao-Bing; Tan, Weihong
• Is Part Of: Chemical science. Volume 8:Issue 12(2017)
• Page Start: 8214
• Page End: 8220
• Publication Date: 2017-10-17
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c7sc03977h
• Languages: English
• ISSNs: 2041-6520

Abstract

Abstract : A two photon-excited fluorescent supramolecular nanoplatform is first designed for quantitative analysis with host molecules, sensing probes and an internal reference. Abstract : Fluorescence quantitative analyses for vital biomolecules are in great demand in biomedical science owing to their unique detection advantages with rapid, sensitive, non-damaging and specific identification. However, available fluorescence strategies for quantitative detection are usually hard to design and achieve. Inspired by supramolecular chemistry, a two-photon-excited fluorescent supramolecular nanoplatform (TPSNP ) was designed for quantitative analysis with three parts: host molecules (β-CD polymers), a guest fluorophore of sensing probes (Np–Ad) and a guest internal reference (NpRh–Ad). In this strategy, theTPSNP possesses the merits of (i) improved water-solubility and biocompatibility; (ii) increased tissue penetration depth for bioimaging by two-photon excitation; (iii) quantitative and tunable assembly of functional guest molecules to obtain optimized detection conditions; (iv) a common approach to avoid the limitation of complicated design by adjustment of sensing probes; and (v) accurate quantitative analysis by virtue of reference molecules. As a proof-of-concept, we utilized the two-photon fluorescent probe NHS–Ad-basedTPSNP-1 to realize accurate quantitative analysis of hydrogen sulfide (H2 S), with high sensitivity and good selectivity in live cells, deep tissues and ex vivo -dissected organs, suggesting that theTPSNP is an ideal quantitative indicator for clinical samples. What's more, TPSNP will pave the way for designing and preparing advanced supramolecular sensors for biosensing and biomedicine.