Visualization of long-term Mg2+ dynamics in apoptotic cells using a novel targetable fluorescent probe. Issue 12 (20th October 2017)

• Record Type: Journal Article
• Authors: Matsui, Yusuke; Funato, Yosuke; Imamura, Hiromi; Miki, Hiroaki; Mizukami, Shin; Kikuchi, Kazuya
• Is Part Of: Chemical science. Volume 8:Issue 12(2017)
• Page Start: 8255
• Page End: 8264
• Publication Date: 2017-10-20
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c7sc03954a
• Languages: English
• ISSNs: 2041-6520

Abstract

Abstract : Long-term Mg 2+ imaging during apoptosis using a HaloTag-coupled Mg 2+ probe demonstrated a Mg 2+ concentration increase caused by dissociation of Mg 2+ from ATP. Abstract : Mg 2+ plays important roles in many physiological processes. However, the underlying molecular mechanisms, especially in the apoptotic pathway, remain unclear due to the diffusion of Mg 2+ probes, which hinders long-term imaging in specific organelles. We developed an immobilized Mg 2+ probe, MGH, which is covalently conjugated with the HaloTag protein in various organelles. HaloTag-coupled MGH enabled long-term imaging of intracellular local Mg 2+ dynamics for 24 h. To exploit this remarkable property, MGH was applied to the investigation of intracellular Mg 2+ dynamics during apoptosis. Time-lapse imaging revealed an increase in the Mg 2+ concentration after apoptotic cell shrinkage. Combined imaging analyses of intracellular Mg 2+ and ATP concentrations strongly suggested that this Mg 2+ concentration increase was caused by the dissociation of Mg 2+ from ATP, along with a decrease in the intracellular ATP concentration. Thus, this protein-coupled Mg 2+ probe could be a new chemical tool to elucidate intracellular Mg 2+ dynamics with high spatiotemporal resolution.