Comparison of cellular and transcriptomic effects between electronic cigarette vapor and cigarette smoke in human bronchial epithelial cells. (December 2017)

• Record Type: Journal Article
• Authors: Anthérieu, Sébastien; Garat, Anne; Beauval, Nicolas; Soyez, Mélissa; Allorge, Delphine; Garçon, Guillaume; Lo-Guidice, Jean-Marc
• Is Part Of: Toxicology in vitro. Volume 45:Part 3(2017)
• Page Start: 417
• Page End: 425
• Publication Date: 2017-12
• Subjects: ALI air-liquid interface -- BT blond tobacco flavored e-liquid -- CM chlorophyll mint flavored e-liquid -- CS cigarette smoke -- E-cig electronic cigarette -- E-vapor electronic vapor -- F − unflavored e-liquid -- GSH reduced glutathione -- GSSG oxidized glutathione -- N − e-liquid without nicotine -- N + e-liquid with 16 mg/mL nicotine; E-cigarette -- Vapors -- BEAS-2B cell line -- Toxicity -- Transcriptomic; Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2016.12.015
• Languages: English
• ISSNs: 0887-2333

Abstract

Abstract: The use of electronic cigarette (e-cig) can be considered as an alternative to smoking. However, due to a lack of thorough toxicological studies, absolute safety of these products cannot be guaranteed. The aim of this in vitro work was to investigate the potential toxicity of e-vapors generated by a smoking machine in human bronchial epithelial BEAS-2B cells cultured at air-liquid interface, in comparison to cigarette smoke (CS). Although CS decreased strongly cell viability from 48 min exposure, e-vapors induced no cytotoxicity up to 288 min exposure. Moreover, oxidative stress was evidenced only after exposure to CS, with a decrease secretion of GRO-ɑ from 8 min and of IL-8 and MCP-1 after 48 min exposure. Only a low increase of IL-6 secretion was measured in cells exposed to e-vapors. Finally, transcriptomic data of exposed cells indicated that a large number of genes were deregulated in response to CS, especially genes involved in important biological functions as oxidative stress and cell death, while e-vapors elicited very discrete modulation. These results strongly suggest a lower toxicity of e-vapors compared to CS in the BEAS-2B cell line and constitute a baseline for further experimental studies with a larger spectrum of e-liquids and e-cig models. Highlights: Original experimental strategy of repeated exposure to e-vapor in ALI-cultured cells Better knowledge on the toxicological effects induced by e-vapors Lower toxicity of e-vapors compared to cigarette smoke in BEAS-2B cells Discrete modulation of the transcriptome of cells exposed to e-vapors Promising tool for regulatory toxicological studies of e-cig