Cytotoxic cycloartane triterpenoids from the leaves of Markhamia stipulata var. canaense. (December 2017)

• Record Type: Journal Article
• Authors: Ngo, Trong Nghia; Phan, Nhat Minh; Bui, Trong Dat; Dang, Van Son; Tran, Cong Luan; Mai, Dinh Tri; Nguyen, Tan Phat
• Is Part Of: Phytochemistry letters. Volume 22(2017:Dec.)
• Page Start: 251
• Page End: 254
• Publication Date: 2017-12
• Subjects: Markhamia stipulata -- Bignoniaceae -- Markhacanasin A -- Markhacanasin B; Botanical chemistry -- Periodicals; Chimie végétale -- Périodiques; 572.205
• DOI: 10.1016/j.phytol.2017.10.020
• Languages: English
• ISSNs: 1874-3900

Abstract

Graphical abstract: Highlights: The cytotoxic property of ethyl acetate fractions of Markhamia stipulata var. canaense V.S. Dang leaves were screened. From the most cytotoxic fraction, two new cycloartane-type triterpenoids (1, 2) were isolated. Their structures were elucidated by IR, UV, HR-ESI–MS, NMR 1D and 2D experiments. Their cytotoxicity activity against five human cancer cell lines (HeLa, HepG2, MCF-7, Jurkat and NCI-H460) were evaluated by SRB assay. Abstract: From the most cytotoxic fraction of the ethyl acetate extract of Markhamia stipulata var. canaense V.S. Dang leaves, two new cycloartane-type triterpenoids, named Markhacanasin A (1 ) and Markhacanasin B (2 ); were isolated by various chromatographic methods Their structures were elucidated by IR, UV, HR-ESI–MS, NMR (1D & 2D) experiments. The cytotoxicity of two new compounds against five human cancer cell lines (HeLa, HepG2, MCF-7, Jurkat and NCI-H460) were evaluated by SRB assay. As results, 1 exhibited significant cytotoxic activity against all cancer cell lines (IC50 ranged from 14.72 to 29.55 μM) while2 did not show activity.