Effect of IGF-1 and Uncultured Autologous Bone-Marrow-Derived Mononuclear Cells on Repair of Osteochondral Defect in Rabbits. (January 2014)

• Record Type: Journal Article
• Authors: Tiwary, Ramesh; Amarpal, ; Aithal, Hari Prasad; Kinjavdekar, Prakash; Pawde, Abhijit M.; Singh, Rajendra
• Is Part Of: Cartilage. Volume 5:Number 1(2014)
• Page Start: 43
• Page End: 54
• Publication Date: 2014-01
• Subjects: articular cartilage -- bone-marrow-derived mononuclear cells -- rabbits -- insulin like growth factor-1; Cartilage -- Periodicals; 616.7
• DOI: 10.1177/1947603513499366
• Languages: English
• ISSNs: 1947-6035

Abstract

Objective: To investigate the utility of bone-marrow-derived mononuclear cells (BMNCs) and insulin like growth factor-1 (IGF-1) in articular cartilage repair. Design : An osteochondral defect of 3 mm diameter and 5 mm depth was created in patellar groove of the left knee joint in each of 36 New Zealand White rabbits. The defect was filled with RPMI-1640 medium in group A (control), autologous BMNCs in group B, and autologous BMNCs plus IGF-1 in group C ( n = 12). Healing of the defect was assessed by gross, scanning electron microscopic, radiographic, and histological examinations up to 90 days. Results : Gross and scanning electron microscopic examination of the healing site revealed superior gross morphology and surface architecture of the healing tissue in the animals of group C as compared to other groups. Radiographically on day 90, the defect area was not distinguishable from the surrounding area in group C, but a small circular defect area was still evident in groups A and B. The regenerated tissue was mostly hyaline in group C and fibrocartilage in groups A and B. The cells were well organized and showed better deposition of proteoglycans in groups C and B than in group A. Conclusions : It was concluded that implantation of bone-marrow-derived nucleated cells may facilitate the healing of osteochondral defects; however, the combination of BMNCs and IGF-1 induces faster and histologically better healing than the BMNCs alone.