An examination of surface epithelium structures of the embryo across the genus Poeciliopsis (Poeciliidae). (28th August 2017)

• Record Type: Journal Article
• Authors: Panhuis, Tami M.; Fris, Megan; Tuhela, Laura; Kwan, Lucia
• Is Part Of: Journal of morphology. Volume 278:Number 12(2017)
• Page Start: 1726
• Page End: 1738
• Publication Date: 2017-08-28
• Subjects: matrotrophy -- placenta morphology -- viviparity; Morphology -- Periodicals; Physiology -- Periodicals; Anatomy -- Periodicals; 571.3
• DOI: 10.1002/jmor.20745
• Languages: English
• ISSNs: 0362-2525

Abstract

Abstract: In viviparous, teleost fish, with postfertilization maternal nutrient provisioning, embryonic structures that facilitate maternal‐fetal nutrient transfer are predicted to be present. For the family Poeciliidae, only a handful of morphological studies have explored these embryonic specializations. Here, we present a comparative morphological study in the viviparous poeciliid genus, Poeciliopsis . Using microscopy techniques, we examine the embryonic surface epidermis of Poeciliopsis species that vary in their level of postfertilization maternal nutrient provisioning and placentation across two phylogenetic clades and three independent evolutionary origins of placentation. We focus on surface features of the embryo that may facilitate maternal‐fetal nutrient transfer. Specifically, we studied cell apical‐surface morphology associated with the superficial epithelium that covers the body and sac (yolk and pericardial) of embryos at different developmental stages. Scanning electron microscopy revealed common surface epithelial cells across species, including pavement cells with apical‐surface microridges or microvilli and presumed ionocytes and/or mucus‐secreting cells. For three species, in the mid‐stage embryos, the surface of the body and sac were covered in microvillus epithelium. The remaining species did not display microvillus epithelium at any of the stages examined. Instead, their epithelium of the body and sac were composed of cells with apical‐surface microridges. For all species, in the late stage embryos, the surface of the body proper was composed of apical‐surface microridges in a "fingerprint‐like arrangement." Despite the differences in the surface epithelium of embryos across Poeciliopsis species and embryonic developmental stages, this variation was not associated with the level of postfertilization maternal nutrient provisioning. We discuss these results in light of previous morphological studies of matrotrophic, teleost fish, phylogenetic relationships of Poeciliopsis species, and our earlier comparative microscopy work on the maternal tissue of the Poeciliopsis placenta. Abstract : Across Poeciliopsis species examined in this study, the epithelial surface features of the embryo, such as the microvillus epithelium shown here, appear to be associated with phylogenetic relationship not level of postfertilization maternal nutrient provisioning.