Low‐level light emitting diode therapy promotes long–term functional recovery after experimental stroke in mice. Issue 12 (2nd May 2017)

• Record Type: Journal Article
• Authors: Lee, Hae In; Lee, Sae‐Won; Kim, Nam Gyun; Park, Kyoung‐Jun; Choi, Byung Tae; Shin, Yong‐Il; Shin, Hwa Kyoung
• Other Names: Luo Qingming guestEditor.
• Is Part Of: Journal of biophotonics. Volume 10:Issue 12(2017)
• Page Start: 1761
• Page End: 1771
• Publication Date: 2017-05-02
• Subjects: Functional recovery -- focal cerebral ischemia -- post-stroke -- BDNF -- neurotrophic factor -- photostimulation; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201700038
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract: We aimed to investigate the effects of low‐level light emitting diode therapy (LED‐T) on the long‐term functional outcomes after cerebral ischemia, and the optimal timing of LED‐T initiation for achieving suitable functional recovery. Focal cerebral ischemia was induced in mice via photothrombosis. These mice were assigned to a sham‐operated (control), ischemic (vehicle), or LED‐T group [initiation immediately (acute), 4 days (subacute) or 10 days (delayed) after ischemia, followed by once‐daily treatment for 7 days]. Behavioral outcomes were assessed 21 and 28 days post‐ischemia, and histopathological analysis was performed 28 days post‐ischemia. The acute and subacute LED‐T groups showed a significant improvement in motor function up to 28 days post‐ischemia, although no brain atrophy recovery was noted. We observed proliferating cells (BrdU + ) in the ischemic brain, and significant increases in BrdU + /GFAP +, BrdU + /DCX +, BrdU + /NeuN +, and CD31 + cells in the subacute LED‐T group. However, the BrdU + /Iba‐1 + cell count was reduced in the subacute LED‐T group. Furthermore, the brain‐derived neurotrophic factor (BDNF) was significantly upregulated in the subacute LED‐T group. We concluded that LED‐T administered during the subacute stage had a positive impact on the long‐term functional outcome, probably via neuron and astrocyte proliferation, blood vessel reconstruction, and increased BDNF expression.Picture : The rotarod test for motor coordination showed that acute and subacute LED‐T improves long‐term functional recovery after cerebral ischemia. Abstract : This study aimed to investigate the effects of low‐level light emitting diode therapy (LED‐T) on the long‐term functional outcomes after cerebral ischemia, and the optimal timing of LED‐T initiation for achieving suitable functional recovery. we clearly found that LED‐T administered during the subacute stage had a positive impact on the long‐term functional outcome, probably via neuron and astrocyte proliferation, blood vessel reconstruction, and increased brain‐derived neurotrophic factor expression.