Cover Feature: Impact of Azidohomoalanine Incorporation on Protein Structure and Ligand Binding (ChemBioChem 23/2017). (21st November 2017)

• Record Type: Journal Article
• Authors: Lehner, Florian; Kudlinzki, Denis; Richter, Christian; Müller‐Werkmeister, Henrike M.; Eberl, Katharina B.; Bredenbeck, Jens; Schwalbe, Harald; Silvers, Robert
• Is Part Of: Chembiochem. Volume 18:Number 23(2017)
• Page Start: 2294
• Page End: 2294
• Publication Date: 2017-11-21
• Subjects: amino acids -- NMR spectroscopy -- proteins -- structure elucidation -- X-ray diffraction; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201700603
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract : The cover feature picture shows the structure of the unnatural amino acid–modified PDZ3 domain, which was thoroughly investigated by NMR spectroscopy and X‐ray crystallography. Azidohomoalanine was introduced by expression in methionine auxotrophic cells to study the impact of the incorporation of an unnatural amino acid on protein structure, dynamics, and ligand binding properties. The results led to the formulation of a flexible guideline that could help researchers that study systems containing unnatural amino acids. More information can be found in the full paper by H. Schwalbe, R. Silvers, et al. onpage 2340 in Issue 23, 2017 (DOI:10.1002/cbic.201700437 ).