Few layered graphene oxide thin films: A potential matrix for immunosensors. Issue 184 (13th October 2017)

• Record Type: Journal Article
• Authors: Pachauri, Namrata; Verma, Shilpi; Singh, Priti; Singh, Surinder P.
• Is Part Of: Integrated ferroelectrics. Issue 184(2017)
• Page Start: 85
• Page End: 91
• Publication Date: 2017-10-13
• Subjects: Graphene oxide -- BSA (bovine serum albumin) -- electrochemical immunosensor; Ferroelectric devices -- Periodicals; Integrated circuits -- Periodicals; 537.244805
• DOI: 10.1080/10584587.2017.1368790
• Languages: English
• ISSNs: 1058-4587

Abstract

ABSTRACT: We have synthesized highly exfoliated graphene oxide (GO) and used it for the fabrication of electrochemical immunosensor. The thin films of GO were spin coated onto ITO/glass substrate and thereafter Bovine Serum Albumin (BSA) antibodies (Ab) were immobilized using EDC-NHS binding chemistry to design immunobioelectrode. The graphene oxide films were characterized using different spectroscopic techniques viz FT-IR, UV-Vis spectroscopy and HRTEM. The electrochemical activity of designed immunoelectrodes was studied by cyclic voltammetry (CV) that revealed a quasi reversible behaviour of graphene oxide films. The differential pulse voltammetry (DPV) measurements of the fabricated immunoelectrodes showed successful detection of analyte at very low concentration ranges (50 fg/mL–100 ng/mL). The present study entails the potential of GO for the development of various immunosensors for different diseases.