Modified extraction and characterization of keratin from Indian goat hoof: A biocompatible biomaterial for tissue regenerative applications. Issue 184 (13th October 2017)

• Record Type: Journal Article
• Authors: Kumar, S. Logesh; Anandhavelu, S.; Sivaraman, J.; Swathy, M.
• Is Part Of: Integrated ferroelectrics. Issue 184(2017)
• Page Start: 41
• Page End: 49
• Publication Date: 2017-10-13
• Subjects: Hoof keratin -- protein -- natural polymer -- intermediate filament -- biocompatibility; Ferroelectric devices -- Periodicals; Integrated circuits -- Periodicals; 537.244805
• DOI: 10.1080/10584587.2017.1368642
• Languages: English
• ISSNs: 1058-4587

Abstract

ABSTRACT: The Goat Hoof Keratin (GHK) is a valuable biodegradable substance for making tissue regeneration. The protein substance from GHK powder is extracted in a clean form by using modified shindai solution technique. Electrophoreical analysis produces polypeptide chains of molecular weight of 40–45 kDa using SDS-PAGE method. FTIR spectra show the characteristic absorption band for α-helix and β-sheet peptide bonds. DSC analysis provides the GHK denaturation occurred at temperature ranging from 200–250°C and thermal degradation occurs at 211°C & 387°C using TGA. The biocompatibility of GHK examined using MTT (3(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay of fibroblast cells shows 91% cell viability.