ABCE1: A special factor that orchestrates translation at the crossroad between recycling and initiation. Issue 10 (3rd October 2017)

• Record Type: Journal Article
• Authors: Mancera-Martínez, Eder; Brito Querido, Jailson; Valasek, Leos Shivaya; Simonetti, Angelita; Hashem, Yaser
• Is Part Of: RNA biology. Volume 14:Issue 10(2017)
• Page Start: 1279
• Page End: 1285
• Publication Date: 2017-10-03
• Subjects: ABCE1 -- cryo-EM -- translation -- recycling -- ribosome; RNA -- Periodicals; Molecular biology -- Periodicals; Molecular biology; RNA; Periodicals; 572.8805
• DOI: 10.1080/15476286.2016.1269993
• Languages: English
• ISSNs: 1547-6286

Abstract

ABSTRACT: For many years initiation and termination of mRNA translation have been studied separately. However, a direct link between these 2 isolated stages has been suggested by the fact that some initiation factors also control termination and can even promote ribosome recycling; i.e. the last stage where post-terminating 80S ribosomes are split to start a new round of initiation. Notably, it is now firmly established that, among other factors, ribosomal recycling critically requires the NTPase ABCE1. However, several earlier reports have proposed that ABCE1 also somehow participates in the initiation complex assembly. Based on an extended analysis of our recently published late-stage 48S initiation complex from rabbit, here we provide new mechanistic insights into this putative role of ABCE1 in initiation. This point of view represents the first structural evidence in which the regulatory role of the recycling factor ABCE1 in initiation is discussed and establishes a corner stone for elucidating the interplay between ABCE1 and several initiation factors during the transit from ribosomal recycling to formation of the elongation competent 80S initiation complex.