An acoustic on-chip goniometer for room temperature macromolecular crystallography. Issue 24 (10th November 2017)

• Record Type: Journal Article
• Authors: Burton, C. G.; Axford, D.; Edwards, A. M. J.; Gildea, R. J.; Morris, R. H.; Newton, M. I.; Orville, A. M.; Prince, M.; Topham, P. D.; Docker, P. T.
• Is Part Of: Lab on a chip. Volume 17:Issue 24(2017)
• Page Start: 4225
• Page End: 4230
• Publication Date: 2017-11-10
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c7lc00812k
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : Development and successful operation of an on-chip goniometer for macromolecular protein crystallography. Abstract : This paper describes the design, development and successful use of an on-chip goniometer for room-temperature macromolecular crystallography via acoustically induced rotations. We present for the first time a low cost, rate-tunable, acoustic actuator for gradual in-fluid sample reorientation about varying axes and its utilisation for protein structure determination on a synchrotron beamline. The device enables the efficient collection of diffraction data via a rotation method from a sample within a surface confined droplet. This method facilitates efficient macromolecular structural data acquisition in fluid environments for dynamical studies.