An ultra-small, multi-point, and multi-color photo-detection system with high sensitivity and high dynamic range. Issue 24 (8th November 2017)

• Record Type: Journal Article
• Authors: Anazawa, Takashi; Yamazaki, Motohiro
• Is Part Of: Lab on a chip. Volume 17:Issue 24(2017)
• Page Start: 4231
• Page End: 4242
• Publication Date: 2017-11-08
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c7lc01070b
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : Fluorescence from four emission points is collimated by four lenses, split into four-color fluxes by four dichroic mirrors, and directly input into the image sensor. Abstract : Although multi-point, multi-color fluorescence-detection systems are widely used in various sciences, they would find wider applications if they are miniaturized. Accordingly, an ultra-small, four-emission-point and four-color fluorescence-detection system was developed. Its size (space between emission points and a detection plane) is 15 × 10 × 12 mm, which is three-orders-of-magnitude smaller than that of a conventional system. Fluorescence from four emission points with an interval of 1 mm on the same plane was respectively collimated by four lenses and split into four color fluxes by four dichroic mirrors. Then, a total of sixteen parallel color fluxes were directly input into an image sensor and simultaneously detected. The emission-point plane and the detection plane (the image-sensor surface) were parallel and separated by a distance of only 12 mm. The developed system was applied to four-capillary array electrophoresis and successfully achieved Sanger DNA sequencing. Moreover, compared with a conventional system, the developed system had equivalent high fluorescence-detection sensitivity (lower detection limit of 17 pM dROX) and 1.6-orders-of-magnitude higher dynamic range (4.3 orders of magnitude).