Proton-detected solid-state NMR detects the inter-nucleotide correlations and architecture of dimeric RNA in microcrystals. Issue 96 (3rd November 2017)

• Record Type: Journal Article
• Authors: Yang, Yufei; Xiang, ShengQi; Liu, Xiaodan; Pei, Xiaojing; Wu, Pengzhi; Gong, Qingguo; Li, Na; Baldus, Marc; Wang, Shenlin
• Is Part Of: Chemical communications. Volume 53:Issue 96(2017)
• Page Start: 12886
• Page End: 12889
• Publication Date: 2017-11-03
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c7cc07483b
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A novel proton-detected solid-state NMR experiment detects the NH⋯N H-bonds within the Watson–Crick base pairs of RNA in crystals. Abstract : We report a novel proton-detected MAS solid-state NMR strategy based on 15 N– 15 N proton assisted recoupling to detect the inter-nucleotide NH⋯N hydrogen bonds within the Watson–Crick base pairs of micro-crystallized dimeric RNA and to confirm the kissing-loop structure. This would contribute to advances in the structural determination of RNA using solid-state NMR.