Kinetically-enhanced DNA detection via multiple-pass exonuclease III-aided target recycling. Issue 24 (21st November 2017)

• Record Type: Journal Article
• Authors: Lee Yu, Henson L.; Zhang, Yinghua; Hsing, I-Ming
• Is Part Of: Analyst. Volume 142:Issue 24(2017)
• Page Start: 4782
• Page End: 4787
• Publication Date: 2017-11-21
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c7an01423f
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A multiple-pass, exonuclease III-mediated target recycling circuit is described for sensitive DNA detection with a shorter assay time. Abstract : One of the promising approaches to address the challenge of detecting dilute nucleic acid analytes is exonuclease III-aided target recycling. In this strategy, the target DNA self-assembles with the reactant DNA probes and displays itself as a reactant and product at the same time. This provides an autonomous mechanism to release and reuse the analyte from each round of reactions for repetitive cycles, which amplifies the signal without amplifying the analyte itself. However, for very low amounts of the analyte, it takes a considerably long time before a detectable signal is generated. Thus, in this paper, we report a kinetically-enhanced target recycling strategy by designing two more target recycling sub-reactions that are triggered by the byproducts of the first reaction involving the target analyte. In this manner, concentrations of up to 0.5 pM of target DNA can be detected in 15 minutes.