Genetic analysis of a PER-2-producing Shewanella sp. strain harbouring a variety of mobile genetic elements and antibiotic resistance determinants. (December 2017)

• Record Type: Journal Article
• Authors: Almuzara, Marisa; Montaña, Sabrina; Lazzaro, Terese; Uong, Sylvia; Parmeciano Di Noto, Gisela; Traglia, German; Bakai, Romina; Centrón, Daniela; Iriarte, Andrés; Quiroga, Cecilia; Ramirez, Maria Soledad
• Is Part Of: Journal of global antimicrobial resistance. Volume 11(2017)
• Page Start: 81
• Page End: 86
• Publication Date: 2017-12
• Subjects: Antibiotic resistance -- Uncommon pathogen -- Shewanella -- blaPER-2 -- Genomic analysis; Drug resistance -- Periodicals; Drug resistance -- Periodicals; Drug resistance; Periodicals; 616.9041
• DOI: 10.1016/j.jgar.2017.06.005
• Languages: English
• ISSNs: 2213-7165

Abstract

Highlights: Description of a novel multidrug-resistant Shewanella sp. recovered from a patient. bla PER-2 -producing Shewanella sp. strain harbouring a novel complex class 1 integron. Horizontal gene transfer could influence survival and adaptability to the hospital environment. Abstract: The objective of this study was to investigate the molecular mechanisms explaining the multidrug-resistant (MDR) phenotype found in a novel clinical Shewanella sp. strain (Shew256) recovered from a diabetic patient. Whole-genome shotgun sequencing was performed using Illumina MiSeq-I and Nextera XT DNA library. De novo assembly was performed with SPAdes. RAST Server was used to predict the open-reading frames and the predictions were confirmed using BLAST. Further genomic analysis was carried out using average nucleotide identity (ANI), ACT (Artemis), OrthoMCL, ARG-ANNOT, ISfinder, PHAST, tRNAscan-SE, plasmidSPAdes, PlasmidFinder and MAUVE. PCR and plasmid extraction were also performed. Genomic analysis revealed a total of 456 predicted genes unique to Shew256 compared with other Shewanella genomes. Moreover, the presence of different resistance genes, including bla PER-2, was found. A complex class 1 integron containing the IS CR1 gene, disrupted by two putative transposase genes, was identified. Furthermore, other resistance genes, a transposon containing aph(3′), insertion sequences, phages and non-coding RNAs were also found. In conclusion, evidence of acquisition of resistance genes and mobile elements that could explain the MDR phenotype were observed. This Shewanella sp. represents a prime example of how antibiotic resistance determinants can be acquired by uncommon pathogens.