Double sex and mab‐3 related transcription factor 1 regulates differentiation and proliferation in dairy goat male germline stem cells. Issue 3 (13th September 2017)

• Record Type: Journal Article
• Authors: Wei, Yudong; Cai, Shufang; Ma, Fanglin; Zhang, Ying; Zhou, Zhe; Xu, Shuanshuan; Zhang, Mengfei; Peng, Sha; Hua, Jinlian
• Is Part Of: Journal of cellular physiology. Volume 233:Issue 3(2018:Mar.)
• Page Start: 2537
• Page End: 2548
• Publication Date: 2017-09-13
• Subjects: Dmrt1 -- goat -- male germline stem cells (mGSCs) -- proliferation -- self‐renewal; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.26129
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract : The protein encoded by double sex and mab‐3 related transcription factor 1 (Dmrt1) gene contains a double sex/mab‐3 domain, which was considered as one of the most conservative structures in sex determination. However, its effect on spermatogenesis of dairy goat spermatogonial stem cells (SSCs) remains to be clarified. For the first time, the roles of Dmrt1 in spermatogenesis of livestock are highlighted. Here, we investigated the expression pattern of Dmrt1 in the testes of dairy goats. Dmrt1 primarily located in undifferentiated SSCs. Moreover, Dmrt1 enhanced differentiation and proliferation of mGSCs. On the contrary, the level of meiosis was down‐regulated, as Dmrt1 determines whether SSCs undergo mitosis and spermatogonial differentiation or meiosis. In the busulfan‐treated mice testes, Dmrt1 repair germ cell damage was emphasized as well. Our results exposed that Dmrt1 maintenance mGSCs in two ways: facilitating proliferation and self‐renewal of SSCs; and reducing the inflammatory response caused by reproductive injury. These findings identify a central role for Dmrt1 in controlling population stability and injury restoring of SSCs. Abstract : For the first time, the roles of Dmrt1 in spermatogenesis of livestock are highlighted. Dmrt1 primarily located in undifferentiated SSCs, moreover, Dmrt1 enhanced differentiation and proliferation of mGSCs. Our results exposed that Dmrt1 maintenance mGSCs in two ways: facilitating proliferation and self‐renewal of SSCs; and reducing the inflammatory response caused by reproductive injury.