Effects of white light‐emitting diode (LED) exposure on retinal pigment epithelium in vivo. Issue 12 (29th June 2017)

• Record Type: Journal Article
• Authors: Jaadane, Imene; Villalpando Rodriguez, Gloria Elisa; Boulenguez, Pierre; Chahory, Sabine; Carré, Samuel; Savoldelli, Michèle; Jonet, Laurent; Behar‐Cohen, Francine; Martinsons, Christophe; Torriglia, Alicia
• Is Part Of: Journal of cellular and molecular medicine. Volume 21:Issue 12(2017)
• Page Start: 3453
• Page End: 3466
• Publication Date: 2017-06-29
• Subjects: light‐emitting diode -- retinal pigment epithelium degeneration -- necrosis -- oxidative stress -- blue light -- blood–retinal barrier; Cytology; Medicine; Molecular Biology; Cytologie -- Périodiques; Médecine -- Périodiques; Biologie moléculaire -- Périodiques; Cytology -- Periodicals; Medicine -- Periodicals; Molecular biology -- Periodicals; 611.01805
• DOI: 10.1111/jcmm.13255
• Languages: English
• ISSNs: 1582-1838

Abstract

Abstract: Ageing and alteration of the functions of the retinal pigment epithelium (RPE) are at the origin of lost of vision seen in age‐related macular degeneration (AMD). The RPE is known to be vulnerable to high‐energy blue light. The white light‐emitting diodes (LED) commercially available have relatively high content of blue light, a feature that suggest that they could be deleterious for this retinal cell layer. The aim of our study was to investigate the effects of "white LED" exposure on RPE. For this, commercially available white LEDs were used for exposure experiments on Wistar rats. Immunohistochemical stain on RPE flat mount, transmission electron microscopy and Western blot were used to exam the RPE. LED‐induced RPE damage was evaluated by studying oxidative stress, stress response pathways and cell death pathways as well as the integrity of the outer blood–retinal barrier (BRB). We show that white LED light caused structural alterations leading to the disruption of the outer blood–retinal barrier. We observed an increase in oxidized molecules, disturbance of basal autophagy and cell death by necrosis. We conclude that white LEDs induced strong damages in rat RPE characterized by the breakdown of the BRB and the induction of necrotic cell death.