Application of dispersive liquid–liquid microextraction for the preconcentration of eight parabens in real samples and their determination by high‐performance liquid chromatography. Issue 22 (26th September 2017)

• Record Type: Journal Article
• Authors: Shen, Xiong; Liang, Jian; Zheng, Luxia; Lv, Qianzhou; Wang, Hong
• Is Part Of: Journal of separation science. Volume 40:Issue 22(2017)
• Page Start: 4385
• Page End: 4393
• Publication Date: 2017-09-26
• Subjects: breast cancer -- design of experiment -- dispersive liquid‐liquid microextraction -- high‐performance liquid chromatography -- parabens; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201700722
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract: A simple and sensitive method for the simultaneous determination of eight parabens in human plasma and urine samples was developed. The samples were preconcentrated using dispersive liquid–liquid microextraction based on the solidification of floating organic drops and determined by high‐performance liquid chromatography with ultraviolet detection. The influence of variables affecting the extraction efficiency was investigated and optimized using Placket–Burman design and Box–Behnken design. The optimized values were: 58 μL of 1‐decanol (as extraction solvent), 0.65 mL methanol (as disperser solvent), 1.5% w/v NaCl in 5.0 mL of sample solution, pH 10.6, and 4.0 min centrifugation at 4000 rpm. The extract was injected into the high‐performance liquid chromatography system for analysis. Under the optimum conditions, the linear ranges for eight parabens in plasma and urine were 1.0–1000 ng/mL, with correlation coefficients above 0.994. The limit of detection was 0.2–0.4 and 0.1–0.4 ng/mL for plasma and urine samples, respectively. Relative recoveries were between 80.3 and 110.7%, while relative standard deviations were less than 5.4%. Finally, the method was applied to analyze the parabens in 98 patients of primary breast cancer. Results showed that parabens existed widely, at least one paraben detected in 96.9% (95/98) of plasma samples and 98.0% (96/98) of urine samples.