Cytogenetic analysis of five recommended and released Philippine varieties of sugarcane (Saccharum officinarum L.) and their parentals. Issue 4 (2nd October 2017)

• Record Type: Journal Article
• Authors: Adajar, Joan Christine O.; Mendioro, Merlyn S.; Villamael, Luisa N.; Engle, Liwayway M.; Manuel, Ma. Carmina C.; Hernandez, Jose E.
• Is Part Of: Caryologia. Volume 70:Issue 4(2017)
• Page Start: 302
• Page End: 323
• Publication Date: 2017-10-02
• Subjects: Meiosis -- chromosomes -- Saccharum officinarum L. -- hybrids -- pollen fertility; Cell nuclei -- Periodicals; Cytogenetics -- Periodicals; Cytology -- Periodicals; 571.66
• DOI: 10.1080/00087114.2017.1352203
• Languages: English
• ISSNs: 0008-7114

Abstract

Abstract: Meiotic chromosomal behavior of five recommended and released Philippine varieties of sugarcane and their parents, namely VMC 95-09 (Q 90 × Unidentified), PSR 97-45 (VMC 74-292 × CP 72-1312), PSR 00-343 (VMC 84-947 × Unidentified), PSR 00-161 (VMC 86-550 × VMC 87-599), and PSR 00-34 (VMC 84-194 × PHIL 89-43) were characterized and analyzed. Samples were collected from the tassel source of Philippine Sugar Research Institute Foundation Inc. (PHILSURIN) located in Negros Occidental, Philippines. Gametic number for each variety was determined at diakinesis and at least 50 cells per meiotic stage was analyzed. All varieties exhibited chromosome mosaicism at diakinesis with n = 40–60. Chromosome clumping was observed at diakinesis of Q 90 (68%), CP 72-1312 (35%), and PSR 97-45 (22%) pollen mother cells. High frequency of bivalent formation (68–100%) was observed, both in parents and hybrids, indicating that chromosome pairing is autosyndetic. Irregularities at meiosis were also observed at high frequencies both in parents and hybrids. Multiple metaphase I plate, non-congression, and laggards were observed at meiosis I and meiosis II. Chromosome contribution of parents to each hybrid was determined based on gametic chromosome number of the parents and their meiotic behavior. Results of this study can be used in identifying best hybrids that can be utilized in an inter or intra-species hybridization to produce better performing sugarcane varieties.