Good cell culture practices & in vitro toxicology. (December 2017)

• Record Type: Journal Article
• Authors: Eskes, Chantra; Boström, Ann-Charlotte; Bowe, Gerhard; Coecke, Sandra; Hartung, Thomas; Hendriks, Giel; Pamies, David; Piton, Alain; Rovida, Costanza
• Is Part Of: Toxicology in vitro. Volume 45:Part 3(2017)
• Page Start: 272
• Page End: 277
• Publication Date: 2017-12
• Subjects: Good cell culture practices -- Good in vitro method practices -- Complex in vitro systems -- Stem-cells -- Good manufacturing practices -- Good laboratory practices; Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2017.04.022
• Languages: English
• ISSNs: 0887-2333

Abstract

Abstract: Good Cell Culture Practices (GCCP) is of high relevance to in vitro toxicology. The European Society of Toxicology In Vitro (ESTIV), the Center for Alternatives for Animal Testing (CAAT) and the In Vitro Toxicology Industrial Platform (IVTIP) joined forces to address by means of an ESTIV 2016 pre-congress session the different aspects and applications of GCCP. The covered aspects comprised the current status of the OECD guidance document on Good In Vitro Method Practices, the importance of quality assurance for new technological advances in in vitro toxicology including stem cells, and the optimized implementation of Good Manufacturing Practices and Good Laboratory Practices for regulatory testing purposes. General discussions raised the duality related to the difficulties in implementing GCCP in an academic innovative research framework on one hand, and on the other hand, the need for such GCCP principles in order to ensure reproducibility and robustness of in vitro test methods for toxicity testing. Indeed, if good cell culture principles are critical to take into consideration for all uses of in vitro test methods for toxicity testing, the level of application of such principles may depend on the stage of development of the test method as well as on the applications of the test methods, i.e., academic innovative research vs. regulatory standardized test method. Highlights: ESTIV 2016 pre-congress session on Good Cell Culture Practices. Good In Vitro Method Practices. Quality assurance for new technological advances in in vitro toxicology. Implementation of a stem cell based assay following GCCP principles. Optimized GLPs/GMPs for conducting in vitro studies for regulatory use.