G-Protein Gα13 Functions with Abl Kinase to Regulate Actin Cytoskeletal Reorganization. Issue 24 (8th December 2017) - Wang, Limin
Abstract: Heterotrimeric G-proteins are essential cellular signal transducers. One of the G-proteins, Gα13, is critical for actin cytoskeletal reorganization, cell migration, cell proliferation, and apoptosis. Previously, we have shown that Gα13 is essential for both G-protein-coupled receptor and receptor tyrosine kinase-induced actin cytoskeletal reorganization such as dynamic dorsal ruffle turnover and cell migration. However, the mechanism by which Gα13 signals to actin cytoskeletal reorganization is not completely understood. Here we show that Gα13 directly interacts with Abl tyrosine kinase, which is a critical regulator of actin cytoskeleton. This interaction is critical for Gα13 -induced dorsal ruffle turnover, endothelial cell remodeling, and cell migration. Our data uncover a new molecular signaling pathway by which Gα13 controls actin cytoskeletal reorganization. Graphical abstract: Highlights: Signaling mechanism by which G-protein Gα13 regulates actin cytoskeletal reorganization was investigated. Gα13 and Abl tyrosine kinase directly interact. Gα13 and Abl regulate the dynamic dorsal ruffle turnover. This interaction between Gα13 and Abl is critical for actin cytoskeletal reorganization.