Hyaluronic acid co-functionalized gold nanoparticle complex for the targeted delivery of metformin in the treatment of liver cancer (HepG2 cells). (5th September 2015)

• Record Type: Journal Article
• Authors: Kumar, C. Senthil; Raja, M.D.; Sundar, D. Sathish; Gover Antoniraj, M.; Ruckmani, K.
• Is Part Of: Carbohydrate polymers. Volume 128(2015)
• Page Start: 63
• Page End: 74
• Publication Date: 2015-09-05
• Subjects: Green synthesis -- Hyaluronic acid -- Gold nanoparticles -- Metformin -- Zebrafish -- HepG2 cells; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2015.04.010
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Green synthesis of AuNPs using extract of Solanum melongena L as a reducing agent. Metformin was successfully loaded on hyaluronic acid capped AuNPs. This formulation revealed better site specific delivery of metformin to HepG2 cells. Abstract: In this study, green synthesis of gold nanoparticles (AuNPs) was achieved using the extract of eggplant as a reducing agent. Hyaluronic acid (HA) serves as a capping and targeting agent. Metformin (MET) was successfully loaded on HA capped AuNPs (H-AuNPs) and this formulation binds easily on the surface of the liver cancer cells. The synthesized nanoparticles were characterized by UV–Vis spectrophotometer, HR-TEM, particle size analyser and zeta potential measurement. Toxicity studies of H-AuNPs in zebra fish confirmed the in vivo safety of the AuNPs. The in vitro cytotoxicity results showed that the amount of MET-H-AuNPs enough to achieve 50% inhibition (IC50 ) was much lower than free MET. Flow cytometry analysis showed the significant reduction in G2/M phase after treatment with MET-H-AuNPs, and molecular level apoptosis were studied using western blotting. The novelty of this study is the successful synthesis of AuNPs with a higher MET loading and this formulation exhibited better targeted delivery as well as increased regression activity than free MET in HepG2 cells.