Genetic engineering as a tool for the generation of mouse models to understand disease phenotypes and gene function. (December 2017)

• Record Type: Journal Article
• Authors: Baumgart, Ann-Kathrin; Beyer, Marc
• Is Part Of: Current opinion in biotechnology. Volume 48(2017)
• Page Start: 228
• Page End: 233
• Publication Date: 2017-12
• Subjects: Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.copbio.2017.06.012
• Languages: English
• ISSNs: 0958-1669

Abstract

Graphical abstract: Highlights: Overview of the current state of the art in genetic engineering of mouse models. Current technical difficulties and future developments for further optimization. Importance of off-target events for the generation of mouse models. Abstract : The usage of mouse models has been vital for biomedical research over the last decades, yet the generation of these models has been extremely difficult and labor-intensive. The identification and generation of nucleases able to introduce site-specific DNA double-strand breaks, particularly the CRIPSR/Cas system, is a major breakthrough for this field as the endogenous DNA repair machinery can be hijacked to specifically introduce genome modifications at these sites. This allows for the time-efficient and cost-efficient generation of mouse models by delivery of designer nucleases together with donor DNA into fertilized oocytes.