An intact gut microbiota may be required for lactoferrin-driven immunomodulation in rats. (December 2017)

• Record Type: Journal Article
• Authors: Wen, Yongping; He, Qiuwen; Ma, Da; Hou, Qiangchuan; Zhang, Heping; Kwok, Lai-Yu
• Is Part Of: Journal of functional foods. Volume 39(2017)
• Page Start: 268
• Page End: 278
• Publication Date: 2017-12
• Subjects: ANOVA analysis of variance -- ELISA enzyme linked-immunosorbent assay -- IFN interferon -- Ig immunoglobulin -- IL interleukin -- Lf lactoferrin -- LPS lipopolysaccharide -- MANOVA multivariate analysis of variance -- OTU operational taxonomic unit -- PCoA principal coordinate analysis -- PCR polymerase chain reaction -- QIIME package Quantitative Insights Into Microbial Ecology package -- RDP Ribosomal Database Project -- SIMPER similarity percentage -- SMRT single-molecule real-time sequencing -- TNF tumor necrosis factor; Lactoferrin -- Immunomodulation -- Gut microbiota -- Single-molecule real-time sequencing -- Dysbiosis; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2017.10.030
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Lactoferrin ingestion shifted the overall fecal microbiota. Prolonged lactoferrin ingestion was required for the fecal microbiota modulation. The gut microbiota might be involved in lactoferrin-induced immunomodulation. Abstract: Lactoferrin can modulate both the host immunity and gut microbiota. However, whether the immune modulation requires the gut microbiota has not been directly shown. Thus, our study compared (1) lactoferrin-driven immunomodulation profiles and (2) changes in fecal phylogenic metagenome with and without antibiotics-induced dysbiosis in rats. Rats receiving only lactoferrin but not both lactoferrin and antibiotics had a Th-1 type cytokine serum profile. Significant differences were detected between the fecal microbiota of the lactoferrin and control groups at day 19 and/or day 33 but not initially, with a shift in the major contributors for community dissimilarity to Clostridium, Lactobacillus, and Oscillibacter valericigenes . The antibiotics-induced dysbiosis enriched the proinflammatory phyla, Proteobacteria and Deferribacteres, together with the anti-inflammatory species, Akkermansia muciniphila, while suppressed some butyrate-producers from the Firmicutes phylum. Our study shows that an intact microbiota is necessary for lactoferrin-driven immunomodulation.