Evaluation of CdSe/ZnS quantum dot–half anti CD3 antibody–conjugate using confocal laser scanning microscopy. (13th August 2017)

• Record Type: Journal Article
• Authors: Udaka, Hikari; Fukuda, Takeshi; Kamata, Norihiko; Suzuki, Miho
• Is Part Of: Molecular crystals and liquid crystals. Volume 653(2017)
• Page Start: 177
• Page End: 181
• Publication Date: 2017-08-13
• Subjects: Antibody -- confocal laser scanning microscopy -- conjugate -- quantum dot -- reduction; Molecular crystals -- Periodicals; Liquid crystals -- Periodicals; Liquid crystals; Molecular crystals; Periodicals; 548
• DOI: 10.1080/15421406.2017.1351283
• Languages: English
• ISSNs: 1542-1406

Abstract

ABSTRACT: We investigated the cellular uptake phenomena of half anti CD3 antibody–CdSe/ZnS quantum dot–conjugate into the live cell by using the confocal laser scanning microscopy. The anti CD3 antibody was efficiently reduced when tris 2–carboxyethyl phosphine hydrochloride was used as a reductant. The molecular weight of obtained half antibody was confirmed by the non–reduction SDS page, and the optimum concentration was 5 mM for obtaining the half antibody. Finally, the confocal laser scanning microscopy image indicates that the CdSe/ZnS quantum dot–conjugate was taken up into the live cell spontaneously by connecting the reduced half antibody around the CdSe/ZnS quantum dot.