Extracellular expression of the HT1 neurotoxin from the Australian paralysis tick in two Saccharomyces cerevisiae strains. (15th December 2017)

• Record Type: Journal Article
• Authors: Karbanowicz, Thomas; Dover, Eric; Mu, Xinyi; Tabor, Ala; Rodriguez-Valle, Manuel
• Is Part Of: Toxicon. Volume 140(2017)
• Page Start: 1
• Page End: 10
• Publication Date: 2017-12-15
• Subjects: Tick -- Toxin -- Yeast surface display -- Neurotoxin -- Holocyclotoxin -- Recombinant; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2017.10.013
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: Surface display libraries (SDL) have predominantly been utilized for the screening of peptides, and single-chain variable IgG fragments, however, the use of SDL for the expression and purification of proteins is gaining interest. Prokaryote SDL express proteins within the periplasm, limiting the application of common screening techniques, such as ELISA and FACS, to assess the viability of recombinant toxin before purification. A previous attempt to express a functional holocyclotoxin-1 (HT1) from the Australian paralysis tick ( Ixodes holocyclus ) using a prokaryotic system was unsuccessful. In this study, the coding sequence (CDS) of HT1 was cloned into the pYD1 plasmid and transformed by electroporation into IMTV014 and EBY100 yeast cell lines. Post induction, recombinant HT1 was identified on the cell surface of IMTV014/ht1 and EBY100/ht1 transformants by FACS, Western blot, and ELISA utilizing dog anti-paralysis tick IgG. The recombinant HT1 was purified, and functionality confirmed by an in vitro synaptosome-binding assay. This research reports for the first time the extracellular expression and display of a functional HT1 on the surface of the S. cerevisiae . It also provides evidence that yeast display libraries provide a viable technology to produce recombinant toxins, and their screening using high throughput methodologies such as FACS. Graphical abstract: Highlights: Paper describes the first expression and display of functional holocyclotoxin-1 on the surface of S. cerevisiae . Yeast displays were screened through high-throughput techniques (FACS). Recombinant holocyclotoxin-1 was purified from the yeast display. Recombinant holocyclotoxin-1 function confirmed through an in vitro synaptosome binding assay.