DOSY NMR and MALDI‐TOF evidence of covalent binding the DNA duplex by trimethylammonium salts of topotecan upon near UV irradiation. (27th May 2015)

• Record Type: Journal Article
• Authors: Naumczuk, Beata; Hyz, Karolina; Kawęcki, Robert; Bocian, Wojciech; Bednarek, Elżbieta; Sitkowski, Jerzy; Wielgus, Ewelina; Kozerski, Lech
• Is Part Of: Magnetic resonance in chemistry. Volume 53:Number 8(2015:Aug.)
• Page Start: 565
• Page End: 571
• Publication Date: 2015-05-27
• Subjects: topotecan -- DNA duplex -- covalent binding -- UV irradiation -- NMR -- MALDI‐TOF MS; Nuclear magnetic resonance spectroscopy -- Periodicals; Chemistry, Organic -- Periodicals; Magnetic resonance -- Periodicals; 538.36
• DOI: 10.1002/mrc.4255
• Languages: English
• ISSNs: 0749-1581

Abstract

Abstract : Using DOSY NMR and MALDI‐TOF MS techniques, we present evidence that quaternary trimethylammonium salts of topotecan, [TPT‐NMe3 ] + X − (X = CF3 SO3, HCOO), bind covalently the natural DNA oligomer upon near UV irradiation in water under physiological conditions. It is shown that formate salt is very reactive at pH 7 and requires short irradiation time. This weak irradiation at 365 nm paves the way for a new application of TPT derivatives in clinical use, which can dramatically increase the therapeutic effects of a medicine. Copyright © 2015 John Wiley & Sons, Ltd. Abstract : Using DOSY NMR and MALDI‐TOF MS techniques, we present evidence that quaternary trimethylammonium salts of Topotecan, [TPT‐NMe3 ] + X − (X = CF3 SO3, HCOO), bind covalently the natural DNA oligomer upon near UV irradiation in water under physiological conditions. This paves the way to a new application of TPT derivatives in clinical use, which can dramatically increase the therapeutic effects of a medicine.