Cofactor Engineering for Enhanced Production of Diols by Klebsiella pneumoniae From Co‐Substrate. Issue 11 (6th September 2017) - Wang, Meng
Abstract : Diols, such as 1, 3‐propanediol (1, 3‐PDO) and 2, 3‐butanediol (2, 3‐BDO), have several promising properties for many synthetic reactions. Here, the cofactor engineering strategy, including the construction of Entner–Doudoroff pathway and transhydrogenase‐based NADH regeneration system, was applied in producing diols from mixtures of glucose and glycerol. Entner–Doudoroff pathway had a high regeneration rate of NAD(P)H. This work described a strategy to administrate intracellular NADH/NAD + ratio and improved the concentration of diols. The improvement of NADH/NAD + ratio also effected gene transcription level of the central carbon pathway and cell growth. Finally, the intracellular NADH/NAD + ratio in KP‐APZDUT was increased by 92.8% compared to the KP‐T and the concentration, yield and productivity of diols were increased to 110.8 g L −1, 0.78 mol mol −1, and 3.46 g Lh −1, respectively. The strategy described here provides an approach to achieve a recombinant strain which is capable of producing diols with high yield and productivity. To the best of our knowledge, the Entner–Doudoroff pathway has not yet been used to produce 1, 3‐PDO or 2, 3‐BDO in Klebsiella pneumoniae . Abstract : Diols, such as 1, 3‐propanediol and 2, 3‐butanediol, have several promising properties for many synthetic reactions. In this study, the cofactor engineering strategy, including the construction of Entner–Doudoroff pathway and transhydrogenase‐based NADH regeneration system, is applied in producing diols from mixtures of glucose and glycerol. The strategy described here provides an approach to achieve a recombinant strain which is capable of producing diols with high yield and productivity. This article is part of an AFOB (Asian Federation of Biotechnology) Special issue. To learn more about the AFOB visitwww.afob.org .