In vivo imaging of the progression of acute lung injury using hyperpolarized [1‐13C] pyruvate. Issue 6 (11th January 2017)

• Record Type: Journal Article
• Authors: Pourfathi, Mehrdad; Xin, Yi; Kadlecek, Stephen J.; Cereda, Maurizio F.; Profka, Harrilla; Hamedani, Hooman; Siddiqui, Sarmad M.; Ruppert, Kai; Drachman, Nicholas A.; Rajaei, Jennia N.; Rizi, Rahim R.
• Is Part Of: Magnetic resonance in medicine. Volume 78:Issue 6(2017)
• Page Start: 2106
• Page End: 2115
• Publication Date: 2017-01-11
• Subjects: hyperpolarization -- DNP -- lungs -- pyruvate -- MRI -- CSI -- MRSI -- carbon‐13 -- lung injury -- ALI -- ARDS; Nuclear magnetic resonance -- Periodicals; Electron paramagnetic resonance -- Periodicals; 616.07548
• DOI: 10.1002/mrm.26604
• Languages: English
• ISSNs: 0740-3194

Abstract

Abstract : Purpose: To investigate pulmonary metabolic alterations during progression of acute lung injury. Methods: Using hyperpolarized [1‐ 13 C] pyruvate imaging, we measured pulmonary lactate and pyruvate in 15 ventilated rats 1, 2, and 4 h after initiation of mechanical ventilation. Lung compliance was used as a marker for injury progression. 5 untreated rats were used as controls; 5 rats (injured‐1) received 1 ml/kg and another 5 rats (injured‐2) received 2 ml/kg hydrochloric acid (pH 1.25) in the trachea at 70 min. Results: The mean lactate‐to‐pyruvate ratio of the injured‐1 cohort was 0.15 ± 0.02 and 0.15 ± 0.03 at baseline and 1 h after the injury, and significantly increased from the baseline value 3 h after the injury to 0.23 ± 0.02 ( P = 0.002). The mean lactate‐to‐pyruvate ratio of the injured‐2 cohort decreased from 0.14 ± 0.03 at baseline to 0.08 ± 0.02 1 h after the injury and further decreased to 0.07 ± 0.02 ( P = 0.08) 3 h after injury. No significant change was observed in the control group. Compliance in both injured groups decreased significantly after the injury ( P < 0.01). Conclusions: Our findings suggest that in severe cases of lung injury, edema and hyperperfusion in the injured lung tissue may complicate interpretation of the pulmonary lactate‐to‐pyruvate ratio as a marker of inflammation. However, combining the lactate‐to‐pyruvate ratio with pulmonary compliance provides more insight into the progression of the injury and its severity. Magn Reson Med 78:2106–2115, 2017. © 2017 International Society for Magnetic Resonance in Medicine.