DNA damage in kidney transplant patients. Role of organ origin. (19th August 2017)

• Record Type: Journal Article
• Authors: Corredor, Zuray; Rodríguez‐Ribera, Lara; Coll, Elisabet; Silva, Irene; Díaz, Juan Manuel; Ballarín, José; Marcos, Ricard; Pastor, Susana
• Other Names: Bonassi S. checker.
• Is Part Of: Environmental and molecular mutagenesis. Volume 58:Number 9(2017)
• Page Start: 712
• Page End: 718
• Publication Date: 2017-08-19
• Subjects: kidney transplantation -- genomic damage -- oxidative damage -- comet assay -- micronucleus assay; Mutagenesis -- Periodicals; Molecular genetics -- Periodicals; Mutagenèse -- Périodiques; Mutagenèse chimique -- Périodiques; Mutation -- Périodiques; Maladies de l'environnement -- Périodiques; Génétique moléculaire -- Périodiques; 576.542
• DOI: 10.1002/em.22117
• Languages: English
• ISSNs: 0893-6692

Abstract

Abstract : Chronic kidney disease (CKD) patients are characterized by elevated levels of genomic damage. This damage increases when kidney function decreases being maximum in hemodialysis patients. As kidney transplantation improves renal function, and it is related with better survival, the aim of our study was to evaluate potential changes in DNA damage levels after kidney transplantation, and comparing living donor recipients with cadaveric donor recipients. The alkaline comet assay was used to determine DNA breaks and oxidative damaged DNA; and the micronucleus assay was used to determine chromosomal breakage and/or aneuploidy. Fifty CKD patients were followed up after 6 and 12 months of their kidney transplantation. All patients increased their genomic damage levels after 6 and 12 months of renal transplantation, compared with those observed before transplantation, despite of the improvement of their metabolic functions. Donor advanced age correlated positively with higher DNA damage. Genomic damage was lower in living donor transplants with respect to cadaveric donor transplants. Our conclusion is that DNA damage increased in kidney transplantation patients, whereas their renal function improved. Higher levels of DNA damage were found in cadaveric donor transplants when compared to living donor transplants. Environ. Mol. Mutagen. 58:712–718, 2017. © 2017 Wiley Periodicals, Inc.