Histidine-alleviated hepatocellular death in response to 4-hydroxynonenal contributes to the protection against high-fat diet-induced liver injury. (December 2017)

• Record Type: Journal Article
• Authors: Song, Qing; Guo, Rui; Wei, Wei; Lv, Lin; Song, Zhenfeng; Feng, Renan; Li, Songtao; Sun, Changhao
• Is Part Of: Journal of functional foods. Volume 39(2017)
• Page Start: 74
• Page End: 83
• Publication Date: 2017-12
• Subjects: 4-HNE 4-hydroxynonenal -- ALT alanine aminotransferase -- AML-12 hepatocyte alpha mouse liver-12 hepatocyte -- AMPK AMP-activated protein kinase -- DCFH-DA 2, 7-dichloro-dihydro-fluorescein diacetate -- DMEM Dulbecco's Modified Eagle Medium -- DMEM/F-12 Dulbecco's Modified Eagle Medium/Ham's Nutrient Mixture F-12, 1:1 -- ERK1/2 extracellular regulated protein kinase1/2 -- FFA free fatty acids -- H&E hematoxylin and eosin -- HFD high-fat diet -- His high dose histidine supplemental group -- His DR HFD-restricted group -- IL-1β interleukin-1beta -- JNK c-Jun-N-terminal kinase -- LDH lactate dehydrogenase -- LSD least significant difference -- MMP mitochondrial membrane potential -- NAC N-acetylcysteine -- NAFLD non-alcoholic fatty liver disease -- NASH nonalcoholic steatohepatitis -- ND normal diet -- NFκB nuclear factor-κB -- p38 MAPK p38 mitogen-activated protein kinase -- PBS phosphate buffered saline -- PKB/Akt protein kinase B -- ROS reactive oxiygen species -- TNF-α tumor necrosis factor alpha; Histidine -- 4-Hydroxynonenal -- ROS -- MAPK -- Liver injury -- NAFLD; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2017.09.056
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Histidine supplementation relieves high-fat diet-induced liver injury. Histidine alleviates hepatocellular death in response to 4-hydroxynonenal. Histidine inhibits 4-hydroxynonenal-activated JNK and p38 MAPK pathways. Histidine inhibits 4-HNE-induced increase of intracellular reactive oxygen species. Histidine blocks 4-HNE-protein adducts formation. Abstract: 4-Hydroxynonenal (4-HNE) is a critical lipid peroxidation product induced by oxidative stress that has been implicated in the pathophysiology of non-alcoholic fatty liver disease (NAFLD). We previously reported that histidine ameliorated oxidative stress in both obese women and high-fat diet (HFD)-induced rats. However, the mechanism of histidine against liver injury in NAFLD remains unclear. This study aimed to investigate the effect of histidine supplementation on alleviating HFD-induced liver injury. Histidine relieved both HFD-induced liver injury and 4-HNE-induced hepatotoxicity. Histidine acted partly by inhibiting both c-Jun-N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) pathways in paralle, while the reactive oxygen species-mediated MAPKs axes were also implicated in the protective role of histidine. Histidine reversed 4-HNE-protein adducts formation in both rat liver and hepatocytes. These findings suggest that increased histidine intake may be a potential therapeutic choice for NAFLD.