Tributyltin exposure increases mortality of nodavirus infected Japanese medaka Oryzias latipes larvae. Issue 2 (30th November 2017)

• Record Type: Journal Article
• Authors: Kitamura, Shin-Ichi; Akizuki, Masaki; Song, Jun-Young; Nakayama, Kei
• Is Part Of: Marine pollution bulletin. Volume 124:Issue 2(2017)
• Page Start: 835
• Page End: 838
• Publication Date: 2017-11-30
• Subjects: Immunotoxicity -- Immune suppression -- Nodavirus -- Oryzias latipes; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2017.02.020
• Languages: English
• ISSNs: 0025-326X

Abstract

Abstract: We investigated the effect of combined exposure to nodavirus infection and TBT on medaka ( Oryzias latipes ). Medaka larvae were infected by immersion in medium containing nodavirus at titers of 10 2.5, 10 3.5, or 10 4.5 TCID50 /mL. Infected fish then were exposed to TBT at 0, 0.17, 0.52, 1.6, or 4.7 μg/L. Of the 12 groups exposed to both stressors, the mortalities of 6 (10 2.5 TCID50 /mL + 0.52, 1.6, or 4.7 μg/L, 10 3.5 TCID50 /mL + 4.7 μg/L and 10 4.5 TCID50 /mL + 1.6 or 4.7 μg/L) were significantly higher than that of each TBT control. Specifically, mortality was 46 ± 5.5% in the group exposed to both 10 2.5 TCID50 /mL virus and 0.52 μg/L TBT, which represent the lowest observed effective dose and concentration, respectively, among the 6 groups with increased mortalities. Our results suggest that combined exposure to both stressors suppresses antiviral mechanisms in the fish, thus increasing mortality. Highlights: The effect of combined nodavirus infection and TBT exposure was assessed in medaka. Mortality was higher in fish exposed to both stressors compared with a single one. Viral LOED was 10 2.5 TCID50 /mL and TBT LOEC was 0.52 μg/L as combined stressors.