Nervous system disruption and swimming abnormality in early-hatched pufferfish (Takifugu niphobles) larvae caused by pyrene is independent of aryl hydrocarbon receptors. Issue 2 (30th November 2017)

• Record Type: Journal Article
• Authors: Itoyama, Tatsuya; Kawara, Moe; Fukui, Makiko; Sugahara, Yuki; Kurokawa, Daisuke; Kawaguchi, Masahumi; Kitamura, Shin-Ichi; Nakayama, Kei; Murakami, Yasunori
• Is Part Of: Marine pollution bulletin. Volume 124:Issue 2(2017)
• Page Start: 792
• Page End: 797
• Publication Date: 2017-11-30
• Subjects: Pyrene -- Aryl hydrocarbon receptor -- β-Naphthoflavone -- Teleosts -- Nervous system -- Behavior; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2017.02.058
• Languages: English
• ISSNs: 0025-326X

Abstract

Abstract: Pyrene, a member of the polycyclic aromatic hydrocarbons (PAHs), contributes to abnormality in the size of the brain and the swimming behavior of pufferfish ( Takifugu niphobles ) larvae. We hypothesized that the aryl hydrocarbon receptor (AHR) may mediate pyrene-induced toxic effects because AHR is assumed to be a candidate for the downstream target of PAHs in many cases. To identify the contribution of AHR on developing pufferfish, we performed exposure experiments using β-naphthoflavone, an agonist of AHR. We found that the toxic effects of pyrene and β-naphthoflavone in pufferfish larvae are fundamentally different. Pyrene specifically induced problems in the developing midbrain and in swimming behavior, while β-naphthoflavone affected the heartbeat rate and the size of the yolk. These results suggest that the behavioral and morphological abnormality caused by pyrene exposure is mediated by an AHR-independent pathway. Alternatively, defects caused by pyrene may be attributed to the inhibition of the FGF signal. Highlights: We examine effects of pyrene and β-naphthoflavone on pufferfish larvae. Pyrene exposed larvae exhibit reduced brain size and abnormal swimming behavior. β-Naphthoflavone affected the heartbeat rate and the size of the yolk. The behavioral and morphological abnormalities caused by pyrene exposure are thought to be mediated by an AHR-independent pathway.