The in vitro comparative study of the effect of BPA, BPS, BPF and BPAF on human erythrocyte membrane; perturbations in membrane fluidity, alterations in conformational state and damage to proteins, changes in ATP level and Na+/K+ ATPase and AChE activities. (December 2017)

• Record Type: Journal Article
• Authors: Maćczak, Aneta; Duchnowicz, Piotr; Sicińska, Paulina; Koter-Michalak, Maria; Bukowska, Bożena; Michałowicz, Jaromir
• Is Part Of: Food and chemical toxicology. Volume 110(2017)
• Page Start: 351
• Page End: 359
• Publication Date: 2017-12
• Subjects: Bisphenols -- Erythrocytes -- Membrane fluidity -- Protein oxidation -- Na+/K+ ATPase -- Acetylcholinesterase; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2017.10.028
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Bisphenols are massively used in the industry, and thus the exposure of biota including humans to these substances has been noted. In this study we have assessed the effect of BPA and its selected analogs, i.e. BPS, BPF and BPAF on membrane of human red blood cells, which is the first barrier that must be overcome by xenobiotics penetrating the cell, and is commonly utilized as a model in the investigation of the effect of different xenobiotics on various cell types. Red blood cells were incubated with BPA and its analogs in the concentrations ranging from 0.1 to 250 μg/ml for 4 h and 24 h. We have noted that the compounds studied altered membrane fluidity at its hydrophobic region, increased internal viscosity and osmotic fragility of the erythrocytes and altered conformational state of membrane proteins. Moreover, bisphenols examined increased thiol groups level, caused oxidative damage to membrane proteins, decreased ATP level, depleted the activity of Na+/K + ATPase and changed the activity of AChE in human red blood cells. It has been shown that the strongest changes were noted in cells treated with BPAF, while BPS caused the weakest (or none) alterations in the parameters studied. Highlights: Effect of BPA, BPS, BPF and BPAF on erythrocyte membrane properties was studied. BPA, BPF and BPAF changed membrane fluidity at its hydrophobic region. Bisphenols caused conformational changes and oxidative damage to proteins. Bisphenols decreased the activity of Na + /K + ATPase and changed the activity of AChE. The strongest changes were induced by BPAF, while the lowest by BPS.