Olive compounds attenuate oxidative damage induced in HEK-293 cells via MAPK signaling pathway. (December 2017)

• Record Type: Journal Article
• Authors: Maalej, Amina; Forte, Maurizio; Bouallagui, Zouhaier; Donato, Stella; Mita, Luigi; Mita, Damiano Gustavo; Filosa, Stefania; Crispi, Stefania; Sayadi, Sami
• Is Part Of: Journal of functional foods. Volume 39(2017)
• Page Start: 18
• Page End: 27
• Publication Date: 2017-12
• Subjects: Olive fruit extract -- Oleuropein -- HEK-293 cells -- Oxidative damage -- Apoptosis -- MAPK pathway; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2017.10.008
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: OFE and Ole markedly attenuate H2 O2 -induced cytotoxicity in HEK-293 cells. OFE and Ole inhibit H2 O2 -ROS and TBARS formation in a dose-dependent manner. OFE and Ole reduce apoptosis through modulating Bcl-2/Bax ratio and MAPK pathways. Abstract: The protective effects of ethanolic olive fruit extract (OFE) and purified oleuropein (Ole) have been evaluated against H2 O2 -induced oxidative damage and apoptosis in human embryonic kidney (HEK-293) cells. Olive compounds demonstrated a protective effect against H2 O2 -induced cell death resulting in total rescue of cell viability. This effect was accompanied by a marked decrease in intracellular reactive oxygen species (ROS) and lipid peroxidation as evidenced by malondialdehyde (MDA) production. Consequently, olive compounds reduced the apoptotic cell death. This was confirmed by flow cytometry analysis for multicaspases and by western blot and real-time PCR analyses for the expression of key effectors involved in the apoptotic process. Our data suggest that the anti-apoptotic effect could be due to the activation of the Erk/p38 signaling pathway and to the modulation of Bcl-2/Bax ratio. These findings provide new insight into the mechanisms underlying the protective effects of OFE and Ole against oxidative damage-induced apoptosis.