Cross-reactivities of mammalian MAPKs antibodies in rotifer and copepod: Application in mechanistic studies in aquatic ecotoxicology. Issue 2 (30th November 2017)

• Record Type: Journal Article
• Authors: Kang, Hye-Min; Jeong, Chang-Bum; Lee, Young Hwan; Cui, Yan-Hong; Kim, Duck-Hyun; Lee, Min-Chul; Kim, Hui-Su; Han, Jeonghoon; Hwang, Dae-Sik; Lee, Su-Jae; Lee, Jae-Seong
• Is Part Of: Marine pollution bulletin. Volume 124:Issue 2(2017)
• Page Start: 614
• Page End: 623
• Publication Date: 2017-11-30
• Subjects: MAPKs -- Rotifer -- Copepod -- Ecotoxicology -- Signal transduction; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2016.11.049
• Languages: English
• ISSNs: 0025-326X

Abstract

Abstract: The mitogen-activated protein kinases (MAPKs) family is known to mediate various biological processes in response to diverse environmental pollutants. Although MAPKs are well characterized and studied in vertebrates, in invertebrates the cross-reactivities of MAPKs antibodies were not clearly known in response to environmental pollutants due to limited information of antibody epitopes with material resources for invertebrates. In this paper, we performed phylogenetic analysis of MAPKs genes in the marine rotifer Brachionus koreanus and the copepods Paracyclopina nana and Tigriopus japonicus . Also in rotifer and copepods, several studies of Western blot of MAPK signaling pathways were shown in response to environmental pollutants, including multi-walled carbon nanotubes (MWCNTs), water-accommodated fractions (WAFs) of crude oil, and microplastics. This paper will provide a better understanding of the underlying mechanistic scenario in terms of cross-reactivities of mammalian antibodies in rotifer and copepod. Highlight: Phylogenetic analysis of MAPKs genes was performed in rotifer and copepods. Western blot of MAPKs were reproducible upon MWCNTs, WAFs, and microplastics exposure.