Slow Intrathecal Injection of rAAVrh10 Enhances its Transduction of Spinal Cord and Therapeutic Efficacy in a Mutant SOD1 Model of ALS. (4th December 2017)

• Record Type: Journal Article
• Authors: Li, Dongxiao; Liu, Chong; Yang, Chunxing; Wang, Dan; Wu, Dongxia; Qi, Yinkuang; Su, Qin; Gao, Guangping; Xu, Zuoshang; Guo, Yansu
• Is Part Of: Neuroscience. Volume 365(2017)
• Page Start: 192
• Page End: 205
• Publication Date: 2017-12-04
• Subjects: ALS amyotrophic lateral sclerosis -- IT intrathecal -- rAAV recombinant adeno-associated virus -- CNS central nervous system -- DRG dorsal root ganglia -- IV intravenous -- qPCR quantitative Polymerase Chain Reaction -- DH dorsal horn -- PF posterior funiculus -- AF anterior funiculus -- cu fasciculus cuneatus -- gr fasciculus gracilis -- GR tuberculum gracile -- CU tuberculum cuneatum -- CSF cerebrospinal fluid; AAV -- gene therapy -- intrathecal -- amyotrophic lateral sclerosis -- SOD1-G93A; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2017.10.001
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: Slow IT injection of rAAV delivers higher transduction to spinal cord compared with the fast injection. Fast IT injection delivers higher transduction to brain and peripheral tissues compared with the slow injection. Slow IT injection of a therapeutic rAAV led to a slightly better outcome in SOD1-G93A mice compared with the fast injection. Injection speed should be taken into consideration in future trials of rAAV gene therapy employing IT injection. Abstract: Mutant SOD1 causes amyotrophic lateral sclerosis (ALS) by a dominant gain of toxicity. Previous studies have demonstrated therapeutic potential of mutant SOD1-RNAi delivered by intrathecal (IT) injection of recombinant adeno-associated virus (rAAV). However, optimization of delivery is needed to overcome the high degree of variation in the transduction efficiency and therapeutic efficacy. Here, on the basis of our previously defined, efficient IT injection method, we investigated the influence of injection speed on transduction efficiency in the central nervous system (CNS). We demonstrate that slow IT injection results in higher transduction of spinal cord and dorsal root ganglia (DRG) while fast IT injection leads to higher transduction of brain and peripheral organs. To test how these effects influence the outcome of RNAi therapy, we used slow and fast IT injection to deliver rAAVrh10-GFP-amiR-SOD1, a rAAV vector that expresses GFP and an artificial miRNA targeting SOD1, in SOD1-G93A mice. Both slow and fast IT injection produced therapeutic efficacy but the slow injection trended slightly toward a better outcome than the fast injection. These results demonstrate that IT injection speed influences the predominance of gene delivery at different CNS sites and should be taken into consideration in future therapeutic trials involving IT injection.