Digital pathology with Fourier ptychography. (June 2015)

• Record Type: Journal Article
• Authors: Horstmeyer, Roarke; Ou, Xiaoze; Zheng, Guoan; Willems, Phil; Yang, Changhuei
• Is Part Of: Computerized medical imaging and graphics. Volume 42(2015)
• Page Start: 38
• Page End: 43
• Publication Date: 2015-06
• Subjects: Digital pathology -- Computational imaging -- Structured illumination -- Super-resolution -- Microscopy; Diagnostic imaging -- Periodicals; Imaging systems in medicine -- Periodicals; Diagnosis, Radioscopic -- Data processing -- Periodicals; Diagnostic Imaging -- Periodicals; Imagerie pour le diagnostic -- Périodiques; Diagnostic imaging; Periodicals; Electronic journals; Electronic journals; 616.0754
• DOI: 10.1016/j.compmedimag.2014.11.005
• Languages: English
• ISSNs: 0895-6111

Abstract

Abstract: Fourier ptychographic microscopy (FPM) is a recently introduced method of acquiring high-resolution, wide field of view (FOV) giga-pixel histology images. The FPM procedure first acquires a sequence of low-resolution images of a sample under variable-angle illumination. It then combines these images using a novel phase retrieval algorithm to improve the employed microscope's resolution beyond its conventional limit. Here, we first describe how FPM's resolution improvement can enhance wide FOV histology imaging. Second, we show that FPM also records a thin sample's optical phase, which can help pathologists digitally extract as much information as possible from a given histology slide.