Alpha7-nicotinic acetylcholine receptors involve the imidacloprid-induced inhibition of IgE-mediated rat and human mast cell activation. Issue 82 (8th November 2017)

• Record Type: Journal Article
• Authors: Shi, Linbo; Xu, Huaping; Wu, Yujie; Li, Xin; Zou, Li; Gao, Jinyan; Chen, Hongbing
• Is Part Of: RSC advances. Volume 7:Issue 82(2017)
• Page Start: 51896
• Page End: 51906
• Publication Date: 2017-11-08
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c7ra07862e
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Although our recent study indicated that imidacloprid, a widely used neonicotinoid insecticide, inhibited IgE-mediated mast cell activation, the inhibition mechanism still remains unclear. Abstract : Although our recent study indicated that imidacloprid, a widely used neonicotinoid insecticide, inhibited IgE-mediated rat mast cell RBL-2H3 activation, little information is available on the relationship between imidacloprid and IgE-mediated human mast cell activation, and the inhibition mechanism still remains unclear. In the present work, the IgE-sensitized RBL-2H3 cells and human basophilic cell KU812 were incubated with imidacloprid or methyllycaconitine (MLA, the antagonist of α7-nAChRs) prior to the treatment of imidacloprid, followed by challenging the cells with dinitrophenyl–human serum albumin and β-lactoglobulin, respectively. The allergic mediator release, Ca 2+ influx in cells, cPLA2 activity, the phosphorylation contents of PLC-γ and NF-κB in FcεRI signaling pathway were tested. The results indicated that imidacloprid could suppress the production of allergic mediators, Ca 2+ mobilization, cPLA2 activity and the expression of the phosphorylated antibodies of PLC-γ and NF-κB in the RBL-2H3 and KU812 cells. Moreover, an IgE-dependent passive cutaneous anaphylaxis model was used to determine whether α7-nAChRs involved the suppressive effects of imidacloprid in vivo . It was shown that MLA alleviated the imidacloprid-induced inhibition on the absorbance value of vascular extravasation in mice. It is the first time it has been demonstrated that α7-nAChRs involve the inhibitory effects of imidacloprid on the IgE-mediated activation of mast cells.