Impact of omega-3 fatty acids on expression of angiogenic cytokines and angiogenesis by adipose-derived stem cells. (November 2017)

• Record Type: Journal Article
• Authors: Parshyna, Iryna; Lehmann, Susann; Grahl, Katrin; Pahlke, Claudia; Frenzel, Annika; Weidlich, Helmut; Morawietz, Henning
• Is Part Of: Atherosclerosis. Volume 30(2017)
• Page Start: 303
• Page End: 310
• Publication Date: 2017-11
• Subjects: Adipose-derived stem cells -- Omega-3 fatty acids -- Angiogenesis -- Angiopoietin-1 -- IL-6 -- IL-8; ADSC adipose-tissue derived stem cells -- ALna alpha linoleic acid -- Ang-1 angiopoietin-1 -- bFGF basic fibroblast growth factor -- CLA conjugated linoleic acid -- DHA docosahexaenoic acid -- EPA eicosapentaenoic acid -- EDA elaidic acid -- HGF hepatocyte growth factor -- HUVECs human umbilical vein endothelial cells -- IL-6 interleukin-6 -- IL-8 interleukin-8 -- LA linoleic acid -- MSCs mesenchymal stem cells -- MA myristic acid -- OA oleic acid -- PI3K phosphatidylinositol 3-kinase -- PKA protein kinase A -- PKC protein kinase C -- TGF-β transforming growth factor-beta -- VEGF vascular endothelial growth factor -- YWHAZ tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta; Atherosclerosis -- Periodicals; Cardiovascular system -- Diseases -- Periodicals; Periodicals; 616.136
• DOI: 10.1016/j.atherosclerosissup.2017.05.040
• Languages: English
• ISSNs: 1567-5688

Abstract

Abstract: Background and aims: Human adipose-tissue derived stem cells (ADSC) are interesting novel targets in tissue engineering and regenerative medicine with pronounced angiogenic capacities. Furthermore, omega-3 fatty acids have been described to mediate cardioprotective effects, but their role in angiogenesis and vascular regeneration is not well-understood. Here, we analyzed the impact of different omega-3 fatty acids on angiogenesis by ADSCs. Methods: Stem cells were cultured as monolayers or in 3D models, in spheroids embedded in collagen matrix or in co-cultures with human umbilical vein endothelial cells (HUVECs) in the Matrigel™ assay. The angiogenic properties of ADSCs were assessed by their sprouting and paracrine activities, gene expression by RT-PCR, Western blot, and enzyme immunoassay. Results: Stimulation of undifferentiated ADSCs with docosahexaenoic acid (DHA) strongly upregulated angiopoietin-1 mRNA levels up to 4.6 ± 0.3 fold. Furthermore, Il-6 and Il-8 mRNAs were increased 4.2 ± 0.5 fold and 7.1 ± 1.1 fold, respectively. On the other hand, addition of DHA significantly decreased the cumulative sprout length by 2.7 ± 0.8 fold and reduced the total number of sprouts by 2.3 ± 0.9 fold in the in vitro angiogenesis assay. Moreover, excretion of IL-8 into the medium rapidly increased up to 1.7 ± 0.3 fold in response to treatment of ADSCs with DHA. Finally, protein kinase C inhibitor RO-31-8220 abrogated DHA-mediated up-regulation of angiopoietin-1 without significantly affecting ADSCs cell viability. Conclusion: In conclusion, ADSCs might regulate the formation and function of microvascular networks.