1H-NMR-Based Metabolomic Analysis of Cerebrospinal Fluid From Adult Bilateral Moyamoya Disease: Comparison With Unilateral Moyamoya Disease and Atherosclerotic Stenosis. Issue 17 (May 2015)

• Record Type: Journal Article
• Authors: Jeon, Jin Pyeong; Yun, Taeho; Jin, Xing; Cho, Won-Sang; Son, Young-Je; Bang, Jae Seung; Kang, Hyun-Seung; Oh, Chang Wan; Kim, Jeong Eun; Park, Sunghyouk
• Other Names: Chen. Ruoli section editor.
• Is Part Of: Medicine. Volume 94:Issue 17(2015)
• Publication Date: 2015-05
• Subjects: Medicine -- Periodicals; Medicine -- Periodicals; Médecine -- Périodiques; Geneeskunde; Medicine; Periodicals; Periodicals; 610.5
• DOI: 10.1097/MD.0000000000000629
• Languages: English
• ISSNs: 0025-7974

Abstract

Abstract : Abstract: Although metabolomics has been increasingly used to observe metabolic pattern and disease-specific metabolic markers, metabolite profiling for moyamoya disease (MMD) has not yet been done in adults. This study investigated cerebrospinal fluid (CSF) metabolites specific to bilateral MMD (B-MMD) and compared them to those of unilateral MMD (U-MMD) or atherosclerotic stenosis with hydrogen-1 nuclear magnetic resonance spectroscopy to identify metabolic biomarkers associated with MMD in adults. CSF samples of B-MMD (n = 29), U-MMD (n = 11), and atherosclerotic cerebrovascular disease (ACVD) (n = 8) were recruited. Principal component analysis, partial least square discriminant analysis, and orthogonal projections to latent structure discriminant analysis (OPLS-DA) were done for the comparisons. Diagnostic performance was acquired by prediction of 1 left-out sample from the distinction model constructed with the rest of the samples. B-MMD showed an increase in glutamine ( P < 0.001) and taurine ( P = 0.004), and a decrease in glucose ( P < 0.001), citrate ( P = 0.002), and myo-inositol ( P = 0.006) than those in ACVD. U-MMD showed a higher level of glutamine ( P = 0.005) and taurine ( P = 0.034), and a lower level of glutamate ( P < 0.004) than those in ACVD. No difference at the metabolite level was observed between B-MMD and U-MMD. Cross-validation with the OPLS-DA model showed a high accuracy for the prediction of MMD. The results of the study suggest that a metabolomics approach may be helpful in confirming MMD and providing a better understanding of MMD pathogenesis. Elevated glutamine in the CSF may be associated with MMD pathogenesis, which was different from ACVD. Abstract : Supplemental Digital Content is available in the text