Myeloid-related protein-8/14 in acute coronary syndrome. (15th December 2017)

• Record Type: Journal Article
• Authors: Sakuma, Masashi; Tanaka, Atsushi; Kotooka, Norihiko; Hikichi, Yutaka; Toyoda, Shigeru; Abe, Shichiro; Taguchi, Isao; Node, Koichi; Simon, Daniel I.; Inoue, Teruo
• Is Part Of: International journal of cardiology. Volume 249(2017)
• Page Start: 25
• Page End: 31
• Publication Date: 2017-12-15
• Subjects: Myeloid-related protein (MRP)-8/14 -- Acute coronary syndrome -- Inflammation -- Thrombosis -- Leukocyte activation; Cardiology -- Periodicals; Electronic journals; 616.12
• DOI: 10.1016/j.ijcard.2017.09.020
• Languages: English
• ISSNs: 0167-5273

Abstract

Abstract: Background: The alarmin family member myeloid-related protein (MRP)-14 (S100A9), which has been identified by platelet transcriptional profiling as an acute myocardial infarction gene, regulates vascular inflammation and thrombosis. Elevated plasma levels of MRP-8/14 (S100A8/A9) heterodimer predict first and recurrent cardiovascular events. The aim of this study was to elucidate pathophysiological roles of MRP-8/14 in acute coronary syndrome (ACS). Methods and results: In 38 consecutive ACS patients, the MRP-8/14 level in coronary artery blood obtained at thrombus aspiration was higher in 23 patients, in whom aspirated thrombus was confirmed, compared to the 15 patients, in whom it was absent [4.86 (1.95, 8.29) vs 2.94 (1.31, 4.44), P = 0.017]. The MRP-8/14 level was correlated with myeloperoxidase (MPO) level (R 2 = 0.52), but not with soluble P-selectin level (R 2 = 0.0002) in the coronary artery blood. Immunohistochemistry of the aspirated thrombus exhibited that expression of MRP8/14 was co-localized with leukocytes positive for activated Mac-1. Finally, in cultured human umbilical vein endothelial cells, MRP-8/14 increased tissue factor expression. Conclusions: Our findings indicate that MRP-8/14 concentration increases in coronary artery blood in association with thrombus formation in ACS, co-localizes with leukocytes, and is associated with leukocyte activation. MRP-8/14 is positioned as a unique biomarker at the interface of inflammation and thrombosis in ACS.