Overexpression of AtPAD4 in transgenic Brachypodium distachyon enhances resistance to Puccinia brachypodii. (19th September 2017)

• Record Type: Journal Article
• Authors: Wang, B.; Wang, N.; Song, N.; Wang, W.; Wang, J.; Wang, X.; Kang, Z.
• Editors: Franken, P.
• Is Part Of: Plant biology. Volume 19:Number 6(2017)
• Page Start: 868
• Page End: 874
• Publication Date: 2017-09-19
• Subjects: AtPAD4 -- Brachypodium distachyon -- plant defence -- Puccinia brachypodii -- salicylic acid; Botany -- Periodicals; Plants -- genetics -- Periodicals; Plants -- growth & development -- Periodicals; Plant Proteins -- Periodicals; Gene Expression Regulation, Plant -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1111/plb.12616
• Languages: English
• ISSNs: 1435-8603

Abstract

Abstract: Brachypodium distachyon (L.) has recently emerged as a model for temperate grasses for investigating the molecular basis of plant–pathogen interactions. Phytoalexin deficient 4 ( PAD4 ) plays a regulatory role in mediating expression of genes involved in plant defence. In this research, we generated transgenic B. distachyon plants constitutively overexpressing AtPAD4 . Two transgenic B. distachyon lines were verified using PCR and GUS phenotype. Constitutive expression of AtPAD4 in B. distachyon enhanced resistance to Puccinia brachypodii . P . brachypodii generated less urediniospores on transgenic than on wild‐type plants. AtPAD4 overexpression enhanced salicylic acid (SA) levels in B. distachyon ‐infected tissues. qRT‐PCR showed that expression of pathogenesis‐related 1 ( PR1 ) and other defence‐related genes were up‐regulated in transformed B. distachyon following infection with P. brachypodii . Our results indicate that AtPAD4 overexpression in B. distachyon plants led to SA accumulation and induced PR gene expression that reduced the rate of colonisation by P. brachypodii .