SCPP Genes and Their Relatives in Gar: Rapid Expansion of Mineralization Genes in Osteichthyans. Issue 7 (22nd June 2017)

• Record Type: Journal Article
• Authors: Kawasaki, Kazuhiko; Mikami, Masato; Nakatomi, Mitsushiro; Braasch, Ingo; Batzel, Peter; H. Postlethwait, John; Sato, Akie; Sasagawa, Ichiro; Ishiyama, Mikio
• Other Names: Braasch Ingo guestEditor.; Postlethwait John H. guestEditor.
• Is Part Of: Journal of experimental zoology. Volume 328:Issue 7(2017)
• Page Start: 645
• Page End: 665
• Publication Date: 2017-06-22
• Subjects: Developmental biology -- Periodicals; Evolution (Biology) -- Periodicals; Molecular evolution -- Periodicals; Zoology -- Periodicals; Evolution, Molecular -- Periodicals; Developmental Biology -- Periodicals; Zoology -- Periodicals; 591
• DOI: 10.1002/jez.b.22755
• Languages: English
• ISSNs: 1552-5007

Abstract

ABSTRACT: Gar is an actinopterygian that has bone, dentin, enameloid, and ganoin (enamel) in teeth and/or scales. Mineralization of these tissues involves genes encoding various secretory calcium‐binding phosphoproteins (SCPPs) in osteichthyans, but no SCPP genes have been identified in chondrichthyans to date. In the gar genome, we identified 38 SCPP genes, seven of which encode "acidic‐residue‐rich" proteins and 31 encode "Pro/Gln (P/Q) rich" proteins. These gar SCPP genes constitute the largest known repertoire, including many newly identified P/Q‐rich genes expressed in teeth and/or scales. Among gar SCPP genes, six acidic and three P/Q‐rich genes were identified as orthologs of sarcopterygian genes. The sarcopterygian orthologs of most of these acidic genes are involved in bone and/or dentin formation, and sarcopterygian orthologs of all three P/Q‐rich genes participate in enamel formation. The finding of these genes in gar suggests that an elaborate SCPP gene‐based genetic system for tissue mineralization was already present in stem osteichthyans. While SCPP genes have been thought to originate from ancient SPARCL1, SPARCL1L1 appears to be more closely related to these genes, because it established a structure similar to acidic SCPP genes probably in stem gnathostomes, perhaps at about the same time with the origin of tissue mineralization. Assuming enamel evolved in stem osteichthyans, all P/Q‐rich SCPP genes likely arose within the osteichthyan lineage. Furthermore, the absence of acidic SCPP genes in chondrichthyans might be explained by the secondary loss of earliest acidic genes. It appears that many SCPP genes expanded rapidly in stem osteichthyans and in basal actinopterygians. GRAPHICAL ABSTRACT: Various secretory calcium‐binding phosphoprotein (SCPP) genes are involved in mineralization of bone, teeth, scales, and fin rays in osteichthyans. All SCPP genes arose by duplication from a common ancestral gene, and this ancestral gene also arose by gene duplication from a different ancestor. Our investigation of genome sequences in gar and other vertebrates revealed the time range of the origin of SCPP genes and the composition of the earliest SCPP gene.