A monoclonal antibody specific for prophase phosphorylation of histone deacetylase 1: a readout for early mitotic cells. Issue 1 (2nd January 2016)

• Record Type: Journal Article
• Authors: Segré, Chiara V.; Senese, Silvia; Loponte, Sara; Santaguida, Stefano; Soffientini, Paolo; Grigorean, Gabriela; Cinquanta, Mario; Ossolengo, Giuseppe; Seiser, Christian; Chiocca, Susanna
• Is Part Of: MAbs. Volume 8:Issue 1(2016)
• Page Start: 37
• Page End: 42
• Publication Date: 2016-01-02
• Subjects: antibody -- cell cycle -- checkpoint -- HDAC1 -- HDAC2 -- HDACs -- mitosis -- phosphorylation -- post-translational modifications -- prophase; Monoclonal antibodies -- Therapeutic use -- Periodicals; Monoclonal antibodies -- Periodicals; Antibodies, Monoclonal -- Periodicals; 616.0798
• DOI: 10.1080/19420862.2015.1098795
• Languages: English
• ISSNs: 1942-0862

Abstract

Abstract : Histone deacetylases (HDACs) are modification enzymes that regulate a plethora of biological processes. HDAC1, a crucial epigenetic modifier, is deregulated in cancer and subjected to a variety of post-translational modifications. Here, we describe the generation of a new monoclonal antibody that specifically recognizes a novel highly dynamic prophase phosphorylation of serine 406-HDAC1, providing a powerful tool for detecting early mitotic cells.